| 2026-09-07 | M. Černák | Dukla Praha → Prostějov | Loan |
| 2026-09-03 | F. Mikeš | Prostějov → Neratovice-Byškovice | Loan |
| 2026-09-02 | L. Endl | Karviná → Prostějov | Loan |
| 2026-08-20 | A. Binar | Hradec Králové → Prostějov | Loan |
| 2026-08-06 | A. Imider | Prostějov → Hlučín | Loan |
| 2026-08-04 | Vladimir Smid | Hanácká → Prostějov | Transfer |
| 2026-07-30 | Lukáš Raab | Prostějov → Vítkovice | Loan |
| 2026-07-23 | Š. Jalovičor | Sigma Olomouc → Prostějov | Loan |
| 2026-07-23 | M. Kolarik | Sigma Olomouc II → Prostějov | Loan |
| 2026-07-21 | L. Hrdlička | Prostějov → Uherský Brod | Loan |
| 2026-07-21 | D. Kerl | Sparta Praha → Prostějov | Loan |
| 2026-07-20 | Marek Halda | Pardubice → Prostějov | Loan |
| 2026-07-20 | M. Surzyn | Pardubice → Prostějov | Loan |
| 2026-07-19 | L. Budínský | Prostějov → Hlučín | Transfer |
| 2026-07-19 | E. Okeke | Hradec Králové → Prostějov | Loan |
| 2026-07-18 | L. Budínský | Prostějov → Hlučín | Transfer |
| 2026-07-16 | L. Lahodny | Prostějov → Blansko | Transfer |
| 2026-07-13 | T. Ushiyama | Dukla Banská Bystrica → Prostějov | Free agent |
| 2026-07-12 | T. Ushiyama | Dukla Banská Bystrica → Prostějov | Free agent |
| 2026-07-07 | F. Žák | Prostějov → Hanácká | Free agent |
| 2026-07-06 | F. Žák | Prostějov → Hanácká | Free agent |
| 2026-06-30 | M. Matocha | Prostějov → Sigma Olomouc II | - |
| 2026-06-30 | J. Habusta | Prostějov → Sigma Olomouc II | - |
| 2026-06-30 | P. Voleský | Táborsko → Prostějov | Free agent |
| 2026-06-30 | M. Kejř | Chrudim → Prostějov | Free agent |
| 2026-06-29 | F. Žák | Hanácká → Prostějov | Return from loan |
| 2026-06-29 | Marek Bielan | Frýdek-Místek → Prostějov | Return from loan |
| 2026-06-29 | M. Lutonský | Prostějov → Artis | Return from loan |
| 2026-06-29 | V. Hranoš | Prostějov → Sparta Praha II | Return from loan |
| 2026-06-29 | M. Vanak | Hranice → Prostějov | Return from loan |
| 2026-06-29 | F. Mikeš | Sokol Hostouň → Prostějov | Return from loan |
| 2026-06-29 | D. Hais | Prostějov → Hradec Králové | Return from loan |
| 2026-06-29 | L. Budínský | Hlučín → Prostějov | Return from loan |
| 2026-06-29 | P. Sokol | Prostějov → Artis | Return from loan |
| 2026-06-29 | A. Imider | Polanka nad Odrou → Prostějov | Return from loan |
| 2026-06-29 | M. Jaron | Unie Hlubina → Prostějov | Return from loan |
| 2026-06-29 | E. Karalic | Prostějov → Stupčanica Olovo | Transfer |
| 2026-06-29 | A. Cissé | Hlučín → Prostějov | Return from loan |
| 2026-06-29 | M. Poturnay | Prostějov → Sparta Prague (U17) | Return from loan |
| 2026-06-29 | M. Poturnay | Prostějov → Sparta Praha II | Return from loan |
| 2026-06-29 | A. Dohnálek | Prostějov → Sigma Olomouc | Return from loan |
| 2026-06-29 | D. Kramar | Prostějov → Sigma Olomouc | Return from loan |
| 2026-06-29 | J. Musil | Prostějov → Slovan Liberec | Return from loan |
| 2026-06-29 | V. Zahradnicek | Prostějov → Sigma Olomouc | Return from loan |
| 2026-06-29 | L. Lahodny | Blansko → Prostějov | Return from loan |
| 2026-06-29 | T. Jager | Prostějov → Hodonín | Return from loan |
| 2026-06-29 | S. Polasek | Prostějov → Zlin | Return from loan |
| 2026-06-29 | P. Voleský | Táborsko → Prostějov | Free agent |
| 2026-06-25 | V. Zahradnicek | Prostějov → Sigma Olomouc | Return from loan |
| 2026-03-19 | Marek Bielan | Prostějov → Frýdek-Místek | Loan |
| 2026-03-11 | A. Cissé | Prostějov → Hlučín | Loan |
| 2026-02-22 | M. Vanak | Prostějov → Hranice | Loan |
| 2026-02-22 | F. Mikeš | Prostějov → Sokol Hostouň | Loan |
| 2026-02-22 | M. Jaron | Prostějov → Unie Hlubina | Loan |
| 2026-02-11 | A. Dohnálek | Sigma Olomouc → Prostějov | Loan |
| 2026-02-11 | D. Kramar | Sigma Olomouc → Prostějov | Loan |
| 2026-02-11 | V. Zahradnicek | Sigma Olomouc → Prostějov | Loan |
| 2026-02-10 | Martin Trunda | Prostějov → Sigma Olomouc (U19) | - |
| 2026-02-09 | J. Musil | Slovan Liberec → Prostějov | Loan |
| 2026-02-08 | L. Budínský | Prostějov → Hlučín | Loan |
| 2026-02-08 | Dominik Fišer | Plzen → Prostějov | Transfer |
| 2026-02-04 | E. Karalic | Vitez → Prostějov | Transfer |
| 2026-02-04 | M. Poturnay | Sparta Prague (U17) → Prostějov | Loan |
| 2026-02-04 | M. Poturnay | Sparta Praha II → Prostějov | Loan |
| 2026-02-01 | M. Lutonský | Artis → Prostějov | Loan |
| 2026-01-28 | P. Mirvald | Prostějov → Bohemians 1905 | Transfer |
| 2026-01-21 | R. Látal | Prostějov → Brodek u Prerova | Transfer |
| 2026-01-15 | J. Harustak | Prostějov → Baník Ostrava | Transfer |
1D. KerlВратарь · 24
27P. MikulecВратарь · 26
3L. EndlЗащитник · 22
19Dominik FišerЗащитник · 20
21J. KadlecЗащитник · 26
2M. KirschbaumЗащитник · 24
5M. KolarikЗащитник · 19
79Šimon PolášekЗащитник · 21
13M. ŠindelářЗащитник · 34
23M. SurzynЗащитник · 28
4T. UshiyamaЗащитник · 25
8D. BauerПолузащитник · 21
17A. BinarПолузащитник · 19
14D. DziubaПолузащитник · 25
12Marek HaldaПолузащитник · 20
6Š. JalovičorПолузащитник · 20
18M. JaroňПолузащитник · 24
10M. KejřПолузащитник · 24
9J. MatoušekПолузащитник · 26
7M. PolášekПолузащитник · 24
5D. VagnerПолузащитник · 19
22M. ZapletalПолузащитник · 27
29D. AlijagićНападающий · 22
32M. ČernákНападающий · 22
11A. ImranaНападающий · 19
70J. KoudelkaНападающий · 33
15D. KramářНападающий · 22
26E. OkekeНападающий · 19
16P. VoleskýНападающий · 27